γ-变形菌中己糖二酸代谢基因及其调控的比较基因组分析。
Comparative genomic analysis of the hexuronate metabolism genes and their regulation in gammaproteobacteria.
机构信息
State Research Institute of Genetics and Selection of Industrial Microorganisms (GosNIIGenetika), Moscow, Russia.
出版信息
J Bacteriol. 2011 Aug;193(15):3956-63. doi: 10.1128/JB.00277-11. Epub 2011 May 27.
Abstract
The hexuronate metabolism in Escherichia coli is regulated by two related transcription factors from the FadR subfamily of the GntR family, UxuR and ExuR. UxuR controls the d-glucuronate metabolism, while ExuR represses genes involved in the metabolism of all hexuronates. We use a comparative genomics approach to reconstruct the hexuronate metabolic pathways and transcriptional regulons in gammaproteobacteria. We demonstrate differences in the binding motifs of UxuR and ExuR, identify new candidate members of the UxuR/ExuR regulons, and describe the links between the UxuR/ExuR regulons and the adjacent regulons UidR, KdgR, and YjjM. We provide experimental evidence that two predicted members of the UxuR regulon, yjjM and yjjN, are the subject of complex regulation by this transcription factor in E. coli.
摘要
大肠杆菌的己糖酸盐代谢受 GntR 家族 FadR 亚家族的两个相关转录因子 UxuR 和 ExuR 调控。UxuR 控制 D-葡萄糖酸盐代谢，而 ExuR 则抑制所有己糖酸盐代谢相关基因的表达。我们采用比较基因组学方法重建了γ变形菌中的己糖酸盐代谢途径和转录调控网络。我们证明了 UxuR 和 ExuR 的结合基序存在差异，鉴定了 UxuR/ExuR 调控网络的新候选成员，并描述了 UxuR/ExuR 调控网络与相邻调控网络 UidR、KdgR 和 YjjM 之间的联系。我们提供了实验证据表明，UxuR 调控网络的两个预测成员 yjjM 和 yjjN 是该转录因子在大肠杆菌中复杂调控的对象。
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