Apparent lack of gene dosage effect in the PLT assay.

In this paper the results of primed lymphocyte typing (PLT) tests are reported which show the response to HLA-D-specific stimulator and to unrelated cells. The aim was to find out the sensitivity of the PLT method for evaluating not only qualitative HLA-D-region differences but also gene dosage effects. In addition, the primed cells were restimulated with cells from HLA-D-homozygous individuals mutually negative with the specific stimulator in the usual 5-day primary mixed lymphocyte culture incubation. No gene dosage effect in PLT experiments could be observed.