Hipp S S, Rockwood L D, Gaafar H A, Han Y
J Clin Microbiol. 1981 Jan;13(1):135-8. doi: 10.1128/jcm.13.1.135-138.1981.
Urine specimens to be tested for Ureaplasma urealyticum (T-strain mycoplasma) must often be transported to a central laboratory for identification. To examine survival of these organisms over 2 days of transport, fresh urine samples were tested for the presence of ureaplasmas and then divided into four groups: (i) fresh, (ii) frozen, (iii) preserved with 1% (wt/vol) boric acid at room temperature, and (iv) preserved with 10% dimethyl sulfoxide and frozen. The samples were cultured on both A7 and New York City solid media, and the estimates of survival were compared to that of fresh urine. Less than 10% survival of Ureaplasma was observed in 11 of 14 specimens stored frozen for 2 days without preservation; six specimens lost all organisms. Specimens containing either dimethyl sulfoxide or boric acid showed higher survival rates, although neither method consistently approached the full recovery of the T-strains found with the fresh urine. Ureaplasmas from fresh specimens grew well on both New York City and A7 media; however, the New York City medium proved superior for those preserved with boric acid and for urine samples containing few ureaplasmas. These results indicate that preservation of samples does increase the yield of U. urealyticum from urine samples delayed in transit.
用于检测解脲脲原体（T株支原体）的尿液标本通常必须运送到中心实验室进行鉴定。为了检测这些微生物在2天运输过程中的存活情况，对新鲜尿液样本进行解脲脲原体检测，然后将其分为四组：（i）新鲜组，（ii）冷冻组，（iii）室温下用1%（重量/体积）硼酸保存组，以及（iv）用10%二甲基亚砜保存并冷冻组。将样本在A7和纽约市固体培养基上培养，并将存活情况的评估结果与新鲜尿液的结果进行比较。在14个未经保存而冷冻2天的标本中，有11个标本解脲脲原体的存活率低于10%；6个标本中所有微生物均消失。含有二甲基亚砜或硼酸的标本显示出较高的存活率，尽管两种方法都未能始终如一地达到新鲜尿液中T株的完全回收率。新鲜标本中的解脲脲原体在纽约市和A7培养基上均生长良好；然而，纽约市培养基对于用硼酸保存的标本以及解脲脲原体含量较少的尿液样本表现出更好的效果。这些结果表明，样本保存确实能提高运输延迟的尿液样本中解脲脲原体的检出率。
