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用于在临床材料原代培养物中鉴定解脲脲原体（人T支原体）的鉴别琼脂培养基（A7）。

Differential agar medium (A7) for identification of Ureaplasma urealyticum (human T mycoplasmas) in primary cultures of clinical material.

作者信息

Shepard M C, Lunceford C D

出版信息

J Clin Microbiol. 1976 Jun;3(6):613-25. doi: 10.1128/jcm.3.6.613-625.1976.

DOI:10.1128/jcm.3.6.613-625.1976
PMID:950379
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC274365/
Abstract

A differential agar medium for the identification of Ureaplasma urealyticum in primary cultures of clinical specimens is described. The differential medium (no. A7) is specific for the identification of U. urealyticum and other members of the genus Ureaplasma. Large-colony, classical Mycoplasma and Acholeplasma species and Proteus L colonies are unreactive on this differential medium. The medium incorporates the biochemical principle of the direct spot test for urease in colonies of Ureaplasma and contains added urea and a sensitive indicator of ammonia, manganous sulfate. Ureaplasma colonies on this medium are identified as dark golden-brown or rich deep-brown colonies, in sharp contrast to the light background of the medium, when viewed by direct transmitted illumination under the low power of the microscope.

摘要

本文描述了一种用于在临床标本原代培养物中鉴定解脲脲原体的鉴别琼脂培养基。该鉴别培养基（编号A7）对解脲脲原体及脲原体属的其他成员具有特异性。大菌落、经典支原体和无胆甾原体属物种以及变形杆菌L菌落在此鉴别培养基上无反应。该培养基纳入了脲原体菌落脲酶直接斑点试验的生化原理，并含有添加的尿素和氨的敏感指示剂硫酸锰。当在显微镜低倍镜下通过直接透射光观察时，该培养基上的脲原体菌落被鉴定为深金棕色或浓郁的深褐色菌落，与培养基的浅色背景形成鲜明对比。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b6/274365/e773a5acd6e4/jcm00227-0103-c.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b6/274365/e773a5acd6e4/jcm00227-0103-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b6/274365/c4ce353c5c90/jcm00227-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b6/274365/3cc7d125fc42/jcm00227-0099-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b6/274365/f30a0b71d67e/jcm00227-0099-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b6/274365/676e24d9a00c/jcm00227-0099-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b6/274365/559c2d887657/jcm00227-0099-e.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b6/274365/fe919769062c/jcm00227-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b6/274365/89eea8651859/jcm00227-0102-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b6/274365/543929e8800d/jcm00227-0102-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b6/274365/3ceb5cb335de/jcm00227-0102-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9b6/274365/0d205233b6f0/jcm00227-0103-a.jpg
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1
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2
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J Bacteriol. 1956 Mar;71(3):362-9. doi: 10.1128/jb.71.3.362-369.1956.
3
Strain of mycoplasma associated with human reproductive failure.与人类生殖功能衰竭相关的支原体菌株。
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Diagnostics (Basel). 2021 Mar 9;11(3):479. doi: 10.3390/diagnostics11030479.
4
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Rev Esp Quimioter. 2021 Jun;34(3):169-184. doi: 10.37201/req/014.2021. Epub 2021 Mar 18.
5
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6
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J Pathog. 2018 May 22;2018:9064952. doi: 10.1155/2018/9064952. eCollection 2018.
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8
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