Magee T R, Asai T, Malka D, Kogoma T
Department of Cell Biology, School of Medicine, University of New Mexico, Albuquerque 87131.
EMBO J. 1992 Nov;11(11):4219-25. doi: 10.1002/j.1460-2075.1992.tb05516.x.
Upon induction of the SOS response in Escherichia coli, the mode of initiation of DNA replication is altered such that it can occur in the absence of normally required protein synthesis. This type of DNA replication has been termed induced stable DNA replication (iSDR). We examined the origin usage during iSDR and found that the initiation of iSDR occurs primarily in the oriC and terC regions of the chromosome in a manner completely independent of transcription, translation and DnaA protein. Minichromosomes (oriC plasmids) pOC23 and pOC81 were induced to replicate in the absence of DnaA protein and transcription after SOS induction. The results localized one of the iSDR origin activities in a 596 bp region which includes the minimal oriC.
在大肠杆菌中诱导SOS反应时，DNA复制的起始模式会发生改变，从而使其能够在缺乏正常所需蛋白质合成的情况下发生。这种类型的DNA复制被称为诱导稳定DNA复制（iSDR）。我们研究了iSDR过程中的起始点使用情况，发现iSDR的起始主要发生在染色体的oriC和terC区域，其方式完全独立于转录、翻译和DnaA蛋白。在SOS诱导后，微小染色体（oriC质粒）pOC23和pOC81在没有DnaA蛋白和转录的情况下被诱导复制。结果将其中一个iSDR起始点活性定位在一个596 bp的区域，该区域包括最小oriC。
