Karnes H T, Gudat J C, O'Donnell C M, Winefordner J D
Clin Chem. 1981 Feb;27(2):249-52.
This heterogeneous assay for tobramycin involves fluorescein-labeled tobramycin, which competes with native unlabeled tobramycin for anti-tobramycin binding sites. Bound and free labeled antigen are separated by precipitation with a second antibody. Fluorescence intensity of the resuspended precipitate is inversely proportional to native tobramycin concentration. Background interference was consistently about 10% of the total fluorescence precipitated. Assay sensitivity was sufficient to detect nanogram quantities of tobramycin per assay tube. Correlation coefficients (r) were 0.96 and 0.98 for comparisons of this assay with a microbiological assay and a radioimmunoassay, respectively. Mean analytical recovery was 101% and the CV was less than 10% throughout the therapeutic range.
这种用于妥布霉素的异质性检测方法涉及荧光素标记的妥布霉素，它与天然未标记的妥布霉素竞争抗妥布霉素结合位点。结合的和游离的标记抗原通过用第二抗体沉淀来分离。重悬沉淀物的荧光强度与天然妥布霉素浓度成反比。背景干扰始终约为沉淀总荧光的10%。检测灵敏度足以检测每个检测管中纳克量的妥布霉素。该检测方法与微生物检测法和放射免疫检测法比较的相关系数（r）分别为0.96和0.98。在整个治疗范围内，平均分析回收率为101%，变异系数小于10%。
