Belpaire F M, Hallynck T, Pyck J
Methods Find Exp Clin Pharmacol. 1982;4(4):249-53.
The homogeneous substrate-labeled fluorescent immunoassay for tobramycin in serum was evaluated. Standard curves are linear and reproducible. The within-run coefficient of variation for control samples containing low, medium and high concentrations of tobramycin ranged from 1.9 to 6.5%; the between-run coefficient of variation was 11% for a control sample at low concentration. The accuracy measured on spiked samples was good and comparable to the accuracy measured by a microbiological assay; it was somewhat better than for the radioimmunoassay. For patient samples, the results with this method correlated well (r - 0.914) with results obtained by radioimmunoassay.
对血清中妥布霉素的均相底物标记荧光免疫测定法进行了评估。标准曲线呈线性且可重复。含低、中、高浓度妥布霉素的对照样品批内变异系数在1.9%至6.5%之间；低浓度对照样品的批间变异系数为11%。加标样品的测定准确度良好，与微生物测定法测得的准确度相当；比放射免疫测定法稍好。对于患者样品，该方法的结果与放射免疫测定法的结果相关性良好（r = 0.914）。
