Siadak A W, Nowinski R C
Immunogenetics. 1981;12(1-2):45-58. doi: 10.1007/BF01561650.
We describe a new murine cell-surface alloantigen, provisionally designated Thy-2, which is expressed primarily on thymocytes and brain tissue. Although Thy-2 is also expressed at lower levels on bone-marrow and spleen cells, this antigen does not appear to be present on lymph-node, liver, or red blood cells. Immunoprecipitation of surface-labeled thymocyte extracts from a variety of inbred strains reveals this antigen to be a single polypeptide of 150000 daltons. Quantitative membrane immunofluorescence demonstrates that Thy-2 is a minor cell-surface component which is present on the majority of thymocytes. Mice heterozygous at the Thy-2 locus express approximately 50 percent as much antigen as positive homozygotes. Expression of the Thy-2 alloantigen is controlled by a single semidominant gene located approximately 3 cM to the right of the H-2K locus on chromosome 17.
我们描述了一种新的小鼠细胞表面同种异体抗原，暂命名为Thy-2，它主要在胸腺细胞和脑组织中表达。尽管Thy-2在骨髓细胞和脾细胞中也有较低水平的表达，但在淋巴结、肝脏或红细胞中似乎不存在这种抗原。对来自多种近交系的表面标记胸腺细胞提取物进行免疫沉淀，结果显示这种抗原是一种分子量为150000道尔顿的单一多肽。定量膜免疫荧光表明，Thy-2是一种存在于大多数胸腺细胞上的次要细胞表面成分。在Thy-2位点杂合的小鼠表达的抗原量约为阳性纯合子的50%。Thy-2同种异体抗原的表达由位于17号染色体上H-2K位点右侧约3厘摩处的一个半显性单基因控制。
