酿酒酵母对三甲硫氨酸摄取过程中肽转运与水解的分离
Separation of peptide transport and hydrolysis in trimethionine uptake by Saccharomyces cerevisiae.
作者信息
Parker D D, Naider F, Becker J M
出版信息
J Bacteriol. 1980 Aug;143(2):1066-9. doi: 10.1128/jb.143.2.1066-1069.1980.
Abstract
Intact cells of Saccharomyces cerevisiae 139 hydrolyzed amino acid-p-nitroanilide by an activity similar to that of aminopeptidase II, as well-characterized external peptidase in yeast. In contrast, trimethionine, a model peptide used in transport assays, was not hydrolyzed by this aminopeptidase II-like activity, and the peptidase activity toward this substrate was localized in the soluble fraction of the yeast. We conclude that this tripeptide is taken up by S. cerevisiae intact and rapidly hydrolyzed inside the cell.
摘要
酿酒酵母139的完整细胞通过一种与酵母中特征明确的外肽酶氨基肽酶II相似的活性来水解氨基酸对硝基苯胺。相比之下，用于转运测定的模型肽三蛋氨酸并未被这种类似氨基肽酶II的活性水解，且针对该底物的肽酶活性定位于酵母的可溶部分。我们得出结论，这种三肽被完整的酿酒酵母摄取并在细胞内迅速水解。
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本文引用的文献
1
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J Physiol. 1972 Dec;227(2):377-94. doi: 10.1113/jphysiol.1972.sp010038.
2
Compartmentation of inhibitors of proteinases A and B and carboxypeptidase Y in yeast.酵母中蛋白酶A、蛋白酶B和羧肽酶Y抑制剂的区室化
Biochem Biophys Res Commun. 1974 Oct 8;60(3):1051-7. doi: 10.1016/0006-291x(74)90419-7.
3
Activities and cellular localization of yeast proteases and their inhibitors.酵母蛋白酶及其抑制剂的活性与细胞定位
Biochem Biophys Res Commun. 1974 Oct 23;60(4):1378-83. doi: 10.1016/0006-291x(74)90350-7.
4
5
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Biochim Biophys Acta. 1973 Jan 26;291(2):388-97. doi: 10.1016/0005-2736(73)90491-4.
6
Peptide transport.肽转运
Biomembranes. 1975;7:167-218. doi: 10.1007/978-1-4684-7668-2_7.
7
Recovery of S. cerevisiae a cells from G1 arrest by alpha factor pheromone requires endopeptidase action.
Cell. 1979 Nov;18(3):623-35. doi: 10.1016/0092-8674(79)90117-x.
8
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Eur J Biochem. 1979 Jun;97(1):169-73. doi: 10.1111/j.1432-1033.1979.tb13099.x.
9
Permeabilization of microorganisms by Triton X-100.
Anal Biochem. 1978 Oct 1;90(1):220-33. doi: 10.1016/0003-2697(78)90026-x.
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