小鼠黑色素瘤细胞上胰岛素结合与内化的细胞周期分析。
Cell-cycle analysis of insulin binding and internalization on mouse melanoma cells.
作者信息
Shimizu N, Shimizu Y, Fuller B B
出版信息
J Cell Biol. 1981 Jan;88(1):241-4. doi: 10.1083/jcb.88.1.241.
Abstract
Binding of 125I-labeled insulin to the surface receptors of Cloudman S-91 mouse melanoma cells (CCL 53.1) was studied at various phases (M, G1, S, and G2) in the cell cycle. Insulin-binding activity was persistently present during the cell cycle but the highest activity was noted at the S-phase. The insulin once bound to the cell surface receptors at any phase of the cell cycle was internalized and degraded, presumably through a lysosomal pathway. Insulin-indexing activity of melanoma cells was not affected by melanocyte-stimulating hormone.
摘要
研究了125I标记的胰岛素与Cloudman S-91小鼠黑色素瘤细胞（CCL 53.1）表面受体在细胞周期各阶段（M期、G1期、S期和G2期）的结合情况。胰岛素结合活性在细胞周期中持续存在，但在S期活性最高。在细胞周期的任何阶段，一旦胰岛素与细胞表面受体结合，就会被内化并降解，推测是通过溶酶体途径。黑色素瘤细胞的胰岛素指数活性不受促黑素细胞激素的影响。
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