McManus L M, Nakane P K
J Immunol. 1981 Apr;126(4):1421-7.
The localization of mouse C1q in various mouse tissues was assessed by immunohistochemistry. In all mouse strains examined, C1q was associated with cells in small focal areas of lymph nodes, Peyer's patches, and spleens often near germinal centers. Ultrastructural studies of these foci demonstrated that C1q was present in the endoplasmic reticulum of mononucleated cells that had an extensive cytoplasmic network. Occasionally, small deposits of C1q were observed in the perinuclear space of mononucleated cells that had scanty cytoplasm with few organelles. Extracellular C1q was found at the surface of interdigitating cell processes in these lymphatic foci. In addition to the presence of C1q within lympho-reticular tissues, C1q frequently was detected in renal glomeruli but not in urinary tract epithelium or intestinal epithelium of adult mice; in contrast, C1q was localized in the intestinal epithelium of neonatal mice. C1q was not detected in tissue fibroblasts nor in cultured fibroblasts; however, a subpopulation of mouse adherent peritoneal cells maintained in vitro did contain C1q. The results of these studies provide new information regarding the distribution and, thus, the possible sites of C1q biosynthesis in normal mouse tissues.
通过免疫组织化学评估小鼠C1q在各种小鼠组织中的定位。在所有检测的小鼠品系中，C1q与淋巴结、派尔集合淋巴结和脾脏中小的局灶性区域的细胞相关，这些区域通常靠近生发中心。对这些病灶的超微结构研究表明，C1q存在于具有广泛细胞质网络的单核细胞的内质网中。偶尔，在细胞质稀少且细胞器很少见的单核细胞的核周间隙中观察到少量C1q沉积物。在这些淋巴病灶中，在交错突细胞突起的表面发现了细胞外C1q。除了在淋巴网状组织中存在C1q外，在成年小鼠的肾小球中经常检测到C1q，但在尿道上皮或肠上皮中未检测到；相反，C1q定位于新生小鼠的肠上皮中。在组织成纤维细胞或培养的成纤维细胞中未检测到C1q；然而，体外培养的小鼠贴壁腹膜细胞亚群确实含有C1q。这些研究结果提供了关于正常小鼠组织中C1q生物合成的分布以及可能部位的新信息。
