Rumley A G, Trope A, Rowe D J, Hainsworth I R
Ann Clin Biochem. 1980 Nov;17(6):315-8. doi: 10.1177/000456328001700607.
The results obtained by an enzyme multiplied immunoassay kit, EMIT, for the measurement of serum digoxin concentration were compared with those from two radioimmunoassay kit methods, Immophase and Dac-Cel. Patients' sera were used to study the correlation between the methods, and three quality control sera with low, intermediate, and high digoxin concentrations were used to study individual method precision. The coefficients of correlation between the three methods varied between 0.915 and 0.951 for serum drug concentrations up to 4.5 nmol/l. There were statistically significant differences between the means of the patients' samples for each method. Precision was acceptable for each method: within-batch percentage coefficient of variation (%CV) less than 8, between-batch %CV less than 10, except for Dac-Cel at low concentration %CV = 18.6. The time taken for the analysis of a batch of 10 samples was between 1.5 and 2 hours, depending on the method.
采用酶放大免疫分析试剂盒（EMIT）测定血清地高辛浓度，并与两种放射免疫分析试剂盒方法（Immophase和Dac-Cel）的测定结果进行比较。使用患者血清研究各方法之间的相关性，并使用三种低、中、高地高辛浓度的质量控制血清研究各方法的精密度。对于血清药物浓度高达4.5 nmol/l的情况，三种方法之间的相关系数在0.915至0.951之间。各方法患者样本均值之间存在统计学显著差异。各方法的精密度均可接受：批内变异系数百分比（%CV）小于8，批间%CV小于10，但低浓度时Dac-Cel的%CV = 18.6除外。根据方法不同，分析一批10个样本所需时间在1.5至2小时之间。
