Eriksen P B, Andersen O
Clin Chem. 1979 Jan;25(1):169-71.
We applied the Enzyme Multiplied Immunoassay Technique (EMIT; Syva Corp., Palo Alto, CA) for determination of serum digoxin to the ABA-100 bichromatic analyzer. Assay conditions were almost exactly as prescribed for the manual procedure, but the ABA-100 offers high automation, smaller reagent volumes, and shorter reaction time. Precision studies gave CV's of less than 10%. Sixty patients' samples, analyzed for digoxin by radioimmunoassay and this enzyme immunoassay, gave a correlation (r) of 0.941. Results obtained with the ABA-100 were apparently slightly higher. One kit provides reagents for 250 assays, as compared to 70 assays with the manual procedure. In an emergency situation a result will be available about 60 min after the patient's sample is received; one operator can analyze about 120 samples in 8 h.
我们将用于测定血清地高辛的酶放大免疫分析技术（EMIT；Syva公司，加利福尼亚州帕洛阿尔托）应用于ABA - 100双色分析仪。分析条件几乎与手工操作规定的完全相同，但ABA - 100具有高度自动化、试剂用量少和反应时间短的特点。精密度研究得出的变异系数小于10%。通过放射免疫分析和这种酶免疫分析对60例患者样本进行地高辛分析，相关性（r）为0.941。使用ABA - 100获得的结果明显略高。一套试剂盒可提供250次分析的试剂，而手工操作则为70次分析。在紧急情况下，在收到患者样本约60分钟后即可得到结果；一名操作人员在8小时内可分析约120个样本。
