Reiss C S, Schulman J L
Infect Immun. 1980 Aug;29(2):719-23. doi: 10.1128/iai.29.2.719-723.1980.
M protein of influenza A virus was detected with rabbit antiserum by both indirect immunofluorescence and by antibody plus complement-mediated cytolysis on the cell surfaces of both productively and nonproductively infected cells. In contrast, antiserum to nucleoprotein failed to react with unfixed infected cells, but did bind to fixed infected cells, especially in the perinuclear area. Incorporation of antiserum to M protein in a T-cell-mediated cytotoxicity assay produced almost complete abrogation of lysis of H-2-compatible cells infected with an influenza A virus of a subtype which differed from that used to elicit the cytotoxic T cells. However, the antibody did not significantly block 51Cr release from cells infected with the homotypic type A influenza virus. These observations are in accord with the hypothesis that the cross-reactive cytotoxic T-cell responses seen with cells infected by heterotypic influenza A viruses are due to recognition of a common M protein.
用兔抗血清通过间接免疫荧光法以及抗体加补体介导的细胞溶解法，在有 productive 感染和 nonproductive 感染的细胞表面检测甲型流感病毒的 M 蛋白。相比之下，抗核蛋白血清不能与未固定的感染细胞发生反应，但能与固定的感染细胞结合，尤其是在核周区域。在 T 细胞介导的细胞毒性试验中加入抗 M 蛋白血清，几乎完全消除了对感染了与用于引发细胞毒性 T 细胞的甲型流感病毒亚型不同的甲型流感病毒的 H-2 相容细胞的裂解。然而，该抗体并未显著阻断同源甲型流感病毒感染细胞的 51Cr 释放。这些观察结果与以下假设一致，即感染异型甲型流感病毒的细胞出现的交叉反应性细胞毒性 T 细胞反应是由于识别了共同的 M 蛋白。
