Wingard J R, Dick J D, Merz W G, Sandford G R, Saral R, Burns W H
Infect Immun. 1980 Aug;29(2):808-13. doi: 10.1128/iai.29.2.808-813.1980.
The ability of clinical isolates of Candida albicans and candida tropicalis to invade through normal and damaged gastrointestinal mucosa was determined. Adult mice were treated with either gentamicin or gentamicin and cytarabine. Suspensions of yeast cells (10(7)) were administered through a catheter intraesophageally. Invasion was determined by culturing liver, kidney, and lung tissue from mice sacrificed after 48 h. C. albicans and C. tropicalis were incapable of invading through normal gastrointestinal mucosa in mice treated only with gentamicin. Two isolates of C. tropicalis penetrated the damaged gastrointestinal mucosa in 69% (49 of 71) of mice treated with gentamicin and cytarabine. In contrast, three isolates of C. albicans penetrated he damaged gastrointestinal mucosa in only 23% (14 of 62) of mice. These results suggest that C. tropicalis is more capable of invading through damaged gastrointestinal mucosa than C. albicans. The observations in this mouse model parallel those seen in patients on cytotoxic drugs. Therefore, this model offers a tool for investigation of the pathogenicity of these organisms in a model analogous to the compromised host.
测定了白色念珠菌和热带念珠菌临床分离株穿透正常和受损胃肠道黏膜的能力。成年小鼠分别用庆大霉素或庆大霉素与阿糖胞苷进行处理。将酵母细胞悬液（10⁷）通过导管经食管给药。通过培养48小时后处死的小鼠的肝、肾和肺组织来确定侵袭情况。仅用庆大霉素处理的小鼠中，白色念珠菌和热带念珠菌无法穿透正常胃肠道黏膜。在用庆大霉素和阿糖胞苷处理的小鼠中，两株热带念珠菌在69%（71只中的49只）小鼠中穿透了受损的胃肠道黏膜。相比之下，三株白色念珠菌仅在23%（62只中的14只）小鼠中穿透了受损的胃肠道黏膜。这些结果表明，热带念珠菌比白色念珠菌更有能力穿透受损的胃肠道黏膜。在这个小鼠模型中的观察结果与细胞毒性药物治疗患者的情况相似。因此，这个模型为研究这些微生物在类似于免疫功能低下宿主的模型中的致病性提供了一个工具。
