α特异性对酿酒酵母中mms3突变在紫外线诱变方面的影响。

a/alpha-specific effect on the mms3 mutation on ultraviolet mutagenesis in Saccharomyces cerevisiae.

作者信息

Martin P, Prakash L, Prakash S

出版信息

J Bacteriol. 1981 May;146(2):684-91. doi: 10.1128/jb.146.2.684-691.1981.

DOI:10.1128/jb.146.2.684-691.1981

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC217013/

Abstract

A new gene involved in error-prone repair of ultraviolet (UV) damage has been identified in Saccharomyces cerevisiae by the mms3-1 mutation. UV-induced reversion is reduced in diploids that are homozygous for mms3-1, only if they are also heterozygous (MATa/MAT alpha) at the mating type locus. The mms3-1 mutation has no effect on UV-induced reversion either in haploids or MATa/MATa or MAT alpha/MAT alpha diploids. The mutation confers sensitivity to UV and methyl methane sulfonate in both haploids and diploids. Even though mutation induction by UV is restored to wild-type levels in MATa/MATa mms3-1/mms3-1 or MAT alpha/MAT alpha mms3-1/mms3-1 diploids, such strains still retain sensitivity to the lethal effects of UV. Survival after UV irradiation in mms3-1 rad double mutant combinations indicates that mms3-1 is epistatic to rad6-1 whereas non-epistatic interactions are observed with rad3 and rad52 mutants. When present in the homozygous state in MATa/MAT alpha his1-1/his1-315 heteroallelic diploids, mms3-1 was found to lower UV-induced mitotic recombination.

摘要

通过mms3 - 1突变，在酿酒酵母中鉴定出一个参与紫外线（UV）损伤易错修复的新基因。只有当mms3 - 1纯合的二倍体在交配型位点也是杂合的（MATa/MATα）时，UV诱导的回复突变才会减少。mms3 - 1突变对单倍体或MATa/MATa或MATα/MATα二倍体中的UV诱导回复突变没有影响。该突变使单倍体和二倍体对UV和甲基磺酸甲酯敏感。尽管在MATa/MATa mms3 - 1/mms3 - 1或MATα/MATα mms3 - 1/mms3 - 1二倍体中，UV诱导的突变诱导恢复到了野生型水平，但这些菌株对UV的致死效应仍保持敏感。mms3 - 1 rad双突变组合经UV照射后的存活情况表明，mms3 - 1对rad6 - 1是上位性的，而与rad3和rad52突变体存在非上位性相互作用。当mms3 - 1以纯合状态存在于MATa/MATα his1 - 1/his1 - 315异等位基因二倍体中时，发现它会降低UV诱导的有丝分裂重组。

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本文引用的文献

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Mutants of yeast defective in mutation induced by ultraviolet light.在紫外线诱导的突变中存在缺陷的酵母突变体。

Genetics. 1971 May;68(1):21-33. doi: 10.1093/genetics/68.1.21.

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The effects of three rad genes on UV induced mutation rates in haploid and diploid Saccharomyces cells.三个rad基因对单倍体和二倍体酿酒酵母细胞中紫外线诱导突变率的影响。

Mol Gen Genet. 1975;136(3):261-72. doi: 10.1007/BF00334021.

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The effect of three rad genes on survival, inter- and intragenic mitotic recombination in Saccharomyces. I. UV irradiation without photoreactivation or liquid-holding post-treatment.三个rad基因对酿酒酵母生存、基因间和基因内有丝分裂重组的影响。I. 无光复活或液体保持后处理的紫外线照射。

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Mating signals control expression of mutations resulting from insertion of a transposable repetitive element adjacent to diverse yeast genes.交配信号控制着因一个可转座重复元件插入到多种酵母基因附近而产生的突变的表达。

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