薄层色谱板中的光活化硅酸锌：液体闪烁计数中误差的一个潜在原因。

Photoactivated zinc silicate in thin layer chromatography plates: a potential cause for error in liquid scintillation counting.

作者信息

Tobias B, Strickler R C

出版信息

Steroids. 1981 Feb;37(2):213-21. doi: 10.1016/s0039-128x(81)80019-0.

Abstract

Thin layer chromatography plates impregnated with fluorescent indicator permit easy identification of many compounds by exposure to ultraviolet light. Exposure of zinc silicate treated plates to ultraviolet light (254 nm) for 30 seconds induced photoluminescence which persisted at significant levels for longer than 24 hours. This artifact significantly interfered with radioactivity quantitation in three commercially available liquid scintillation solutions. Chromatography plates impregnated with calcium silicate demonstrated insignificant ultraviolet light induced photoluminescence. The use of 14C rather than 3H-radiolabeled compounds, protecting the plate with aluminum foil when visualizing reference compounds with ultraviolet light, and heating the photoactivated plate to accelerate disappearance of the photoluminescence minimized the error in liquid scintillation counting.

摘要

浸渍有荧光指示剂的薄层色谱板通过暴露于紫外光下可轻松鉴定许多化合物。将硅酸锌处理过的板暴露于紫外光（254纳米）下30秒会诱导光致发光，这种光致发光会在显著水平上持续超过24小时。这种假象在三种市售的液体闪烁溶液中显著干扰了放射性定量。浸渍有硅酸钙的色谱板显示出由紫外光诱导的不显著的光致发光。使用14C而非3H放射性标记的化合物、在用紫外光观察参比化合物时用铝箔保护板以及加热光活化板以加速光致发光的消失，可将液体闪烁计数中的误差降至最低。