Madeira-Lopes A, van Uden N
Z Allg Mikrobiol. 1981;21(1):53-5.
Chloramphenicol decreased the maximum temperature for growth of a petite mutant of Saccharomyces cerevisiae, shifted the ARRHENIUS plot of thermal death to lower temperatures and shortened correspondingly, the ARRHENIUS plot of growth, while an associative thermal profile was maintained. At saturating concentrations (about 5 mg per ml) of chloramphenicol in liquid mineral medium with vitamins and glucose the final maximum temperature for growth was depressed from about 40 degrees C to about 37 degrees C. The results suggested that chloramphenicol acted in the mutant on targets other than mitochondrial ribosomes and that these targets are identical or associated with the death and Tmax sites of the yeast.
氯霉素降低了酿酒酵母一个小菌落突变体生长的最高温度，将热致死的阿累尼乌斯曲线移至较低温度，并相应缩短了生长的阿累尼乌斯曲线，同时保持了相关的热曲线。在含有维生素和葡萄糖的液体矿物培养基中，当氯霉素浓度达到饱和（约每毫升5毫克）时，生长的最终最高温度从约40℃降至约37℃。结果表明，氯霉素在该突变体中作用于线粒体核糖体以外的靶点，并且这些靶点与酵母的死亡位点和最高温度位点相同或相关。
