Ramírez Ramos A, Domínguez N, Makino R, Barrera C
Acta Gastroenterol Latinoam. 1980;10(3):207-12.
We have performed a total of 107 cultures from three critical areas of an Olympus Panendoscope Model GIF-D2 in order to evaluate bacteriologically cur system of desinfection of this endoscope. Samples were taken from the distal end, external surface and biopsy canal before and after an endoscopic examination was performed. The procedure of desinfection employed was as follows: washing of the distal end, external surface and biopsy canal with Hexaclorophel (Phisohex) diluted 50% with water and a second washing with tap water. In the middle of the study, we added a second washing of the biopsy canal with ten ml. of ether alcohol to allow for better drying. As a result of the present study we observed that in the distal end in 50% of the samples we encountered bacteria. Cultures of the external surface were positive in 20% of samples. The biopsy canal should be washed with ether alcohol to allow for complete drying, because when we did not use this method, Pseudomonas Aeruginosa was isolated. After this modification we did not isolate bacteria. The most frequent types of isolated bacteria were from the normal oropharyngeal flora. From the present study we can conclude that desinfection of the Panendespe with Hexaclorophen gives satisfactory results on the external surface of the endoscope. Biopsy canal requires additional washing with ether alcohol. However, both procedures do not assure a satisfactory desinfection of the distal end.
为了从细菌学角度评估 Olympus Panendoscope Model GIF-D2 型内镜的消毒系统，我们对该内镜的三个关键部位总共进行了107次培养。样本在进行内镜检查前后分别取自远端、外表面和活检通道。所采用的消毒程序如下：用50%水稀释的六氯酚（Phisohex）清洗远端、外表面和活检通道，然后用自来水再次清洗。在研究过程中，我们增加了用10毫升乙醚酒精对活检通道进行第二次清洗，以便更好地干燥。本研究结果显示，在远端，50%的样本中发现了细菌。外表面培养20%的样本呈阳性。活检通道应用乙醚酒精清洗以确保完全干燥，因为在未使用此方法时，分离出了铜绿假单胞菌。进行此改进后，未分离出细菌。最常见的分离细菌类型来自正常口咽菌群。从本研究可以得出结论，用六氯酚对该内镜进行消毒在外表面能取得满意结果。活检通道需要用乙醚酒精进行额外清洗。然而，这两个程序都不能确保远端消毒令人满意。
