Krakowka S, Austin N J, Long D J, Helphrey M
Am J Vet Res. 1981 Jan;42(1):149-50.
A revised surgical procedure for derivation of gnotobiotic pups was described. Pregnant bitches (n = 11) were sedated and anesthetized (epidural anesthesia). Fetal pups were removed from the uterine horns and then transferred to the isolation units where they were removed from the sacs and stimulated. The method compares favorably with the previously used hysterectomy procedure.
描述了一种用于获得无菌幼崽的改良手术程序。对11只怀孕母犬进行镇静和麻醉（硬膜外麻醉）。从子宫角取出胎儿幼犬，然后转移到隔离单元，在那里将它们从囊中取出并进行刺激。该方法与先前使用的子宫切除术程序相比具有优势。
