Krakowka S, Long D, Mezza R, Mador R A, Koestner A
Lab Anim Sci. 1978 Jun;28(3):327-30.
Revised techniques for derivation and maintenance of gnotobiotic dogs for research purposes were outlined. Gravid uteri were removed from pregnant beagle bitches and passed through a germicidal trap into a flexible plastic isolation unit. Pups were raised on a milk replacement diet and weaned to solid food by 4 weeks of age.
概述了用于研究目的的无菌犬培育和维持的改良技术。从怀孕的比格犬母犬体内取出妊娠子宫，通过一个杀菌阱进入一个柔性塑料隔离单元。幼犬采用代乳品饲养，4周龄时断奶并开始食用固体食物。
