Kater S B, Galvin N J
J Cell Biol. 1978 Oct;79(1):20-6. doi: 10.1083/jcb.79.1.20.
Three experimental techniques were employed to examine coupling between acinar cells of the mouse salivary gland. Passage of DC current pulses via intracellular microelectrodes between neighboring cells showed that small ions could be directly passed from one cell to another. Intracellular iontophoresis of the dye Lucifer Yellow CH into a single cell indicated that small molecules could spread by means of intercellular cytoplasmic bridges througout an acinus and, occasionally, into cells of adjacent acini. Freeze-fracture replicas of acinar cell membranes indicated the presence of gap junctions which were correlated with both electrical and dye coupling experiments. Suggestions are made for the function of direct intercellular exchange in salivary secretory cells. The role of electrical coupling in coordination of the activity of different secretory cell types is discussed as one possible function.
采用了三种实验技术来检测小鼠唾液腺腺泡细胞之间的耦联。通过细胞内微电极在相邻细胞之间施加直流电流脉冲，结果表明小分子离子能够直接从一个细胞传递到另一个细胞。将染料路西弗黄CH进行细胞内离子导入单个细胞，结果表明小分子能够借助细胞间的胞质桥在整个腺泡中扩散，偶尔还能扩散到相邻腺泡的细胞中。腺泡细胞膜的冷冻断裂复制品显示存在间隙连接，这与电耦联和染料耦联实验相关。文中对唾液分泌细胞中细胞间直接交换的功能提出了建议。讨论了电耦联在不同分泌细胞类型活动协调中的作用，这是一种可能的功能。
