Robinson L K, Hurley L S
J Nutr. 1981 May;111(5):869-77. doi: 10.1093/jn/111.5.869.
The influence of a maternal dietary deficiency of zinc, or food restriction on the content and in vitro secretion of pancreatic hormones was measured in rat fetuses on day 19 of gestation. Pregnant Sprague-Dawley rats were fed a zinc-deficient diet (0.4 +/- 0.1 ppm zinc) ad libitum or a zinc-supplemented control diet (100 ppm zinc) either ad libitum or with restricted intake. The insulin content of the fetal pancreas was lower in fetuses from rats subjected to zinc deprivation or to food restriction than in controls. The lower insulin content of fetal pancreata from zinc-deficient rats was related to a lower than normal proportion of insulin-containing beta cells. The lower insulin content of fetal pancreata from rats subjected to food restriction was related to a less than normal amount of insulin per beta cell. Glucagon concentration in the fetal pancreas was lower than normal in response to zinc deficiency but not to food restriction. Fetal hormone release in vitro was also influenced by the maternal diet. The rate of insulin secretion in vitro was normal in fetuses from zinc-deficient rats, but was accelerated in those from females subjected to calorie restriction. The rate of glucagon secretion in vitro was lower than normal in the pancreata of zinc-deficient fetuses. We conclude that insulin and glucagon levels in the fetus were affected by maternal zinc deficiency and, to a lesser extent, by calorie restriction.
在妊娠第19天，对大鼠胎儿测量母体饮食中锌缺乏或食物限制对胰腺激素含量及体外分泌的影响。将怀孕的斯普拉格-道利大鼠随意喂食缺锌饮食（锌含量为0.4±0.1 ppm）或补充锌的对照饮食（锌含量为100 ppm），要么随意进食，要么限制摄入量。与对照组相比，遭受锌剥夺或食物限制的大鼠所产胎儿的胰腺胰岛素含量较低。缺锌大鼠所产胎儿胰腺胰岛素含量较低与含胰岛素β细胞比例低于正常水平有关。遭受食物限制的大鼠所产胎儿胰腺胰岛素含量较低与每个β细胞胰岛素含量低于正常水平有关。胎儿胰腺中的胰高血糖素浓度因锌缺乏而低于正常水平，但不受食物限制的影响。胎儿激素的体外释放也受母体饮食的影响。缺锌大鼠所产胎儿的体外胰岛素分泌率正常，但热量限制的母鼠所产胎儿的体外胰岛素分泌率加快。缺锌胎儿胰腺的体外胰高血糖素分泌率低于正常水平。我们得出结论，胎儿体内的胰岛素和胰高血糖素水平受母体锌缺乏影响，在较小程度上也受热量限制的影响。
