Seto J T, Garten W, Rott R
Arch Virol. 1981;67(1):19-30. doi: 10.1007/BF01314598.
Cultured cells of the chorioallantoic membrane (CAM) fulfilled the need of using the same cell system that was permissive for representative paramyxoviruses to carry out studies on the biosynthesis of their glycoproteins in infected cells. The polypeptides composition of the respective paramyxoviruses [Newcastle disease virus (NDV), paramyxovirus Yucaipa (PMY), and Sendai virus], grown in eggs and CAM-cells, was essentially identical. In egg-grown PMY a large glycoprotein (LGP) was present but only in some CAM-grown preparations of virus labeled with [3H]-glucosamine and rarely in [35S]-methionine or [3H]-amino acids (valine, leucine, and tyrosine) labeled viruses. The site of cleavage of precursor F0 to F1,2 was not the same. In contrast to the cleavage of Sendai virus glycoprotein, cleavage was intracellular in NDV and PMY infected cells. Homologous antisera against the glycoproteins failed to inhibit cleavage of HN0 or F0 in cells infected with the representative paramyxoviruses.
绒毛尿囊膜（CAM）的培养细胞满足了使用对代表性副粘病毒具有易感性的相同细胞系统来开展其在感染细胞中糖蛋白生物合成研究的需求。在鸡胚和CAM细胞中培养的各副粘病毒（新城疫病毒（NDV）、尤凯帕副粘病毒（PMY）和仙台病毒）的多肽组成基本相同。在鸡胚中培养的PMY存在一种大糖蛋白（LGP），但仅在一些用[3H] - 葡糖胺标记的CAM培养病毒制剂中存在，在用[35S] - 甲硫氨酸或[3H] - 氨基酸（缬氨酸、亮氨酸和酪氨酸）标记的病毒中很少出现。前体F0裂解为F1,2的位点不同。与仙台病毒糖蛋白的裂解不同，NDV和PMY感染细胞中的裂解发生在细胞内。针对这些糖蛋白的同源抗血清未能抑制感染代表性副粘病毒的细胞中HN0或F0的裂解。
