Disteche C M, Carrano A V, Ashworth L K, Burkhart-Schultz K, Latt S A
Cytogenet Cell Genet. 1981;29(4):189-97. doi: 10.1159/000131569.
Cells containing the mouse T(X;7)1Ct translocation were used to separate active and inactive X chromosomes. Cytophotometric data showed that the X(7) chromosome was at least 15% larger than any normal mouse chromosome. Cloned cell lines were established from male and female embryos with a normal karyotype and carrying the X(7) chromosome in an active or inactive state as shown by replication kinetics. As expected from the cytophotometric data, flow histograms of normal mouse chromosomes stained with 33258 Hoechst had five peaks. A sixth peak was present when the chromosomes came from mice carrying T1Ct. In this peak 6, 30 to 50% of the chromosomes were X(7) and there was almost no contamination by the normal X chromosome.
含有小鼠T(X;7)1Ct易位的细胞被用于分离活性和非活性X染色体。细胞光度测定数据表明，X(7)染色体比任何正常小鼠染色体至少大15%。从具有正常核型且携带处于活性或非活性状态的X(7)染色体的雄性和雌性胚胎中建立了克隆细胞系，这通过复制动力学得以证明。正如细胞光度测定数据所预期的那样，用33258 Hoechst染色的正常小鼠染色体的流式直方图有五个峰。当染色体来自携带T1Ct的小鼠时，出现了第六个峰。在这个峰6中，30%至50%的染色体是X(7)，并且几乎没有正常X染色体的污染。
