Wirschubsky Z, Ingvarsson S, Carstenssen A, Wiener F, Klein G, Sümegi J
Proc Natl Acad Sci U S A. 1985 Oct;82(20):6975-9. doi: 10.1073/pnas.82.20.6975.
Experiments have been carried out to establish the relative position of the mouse Ig heavy chain locus and the c-myc oncogene. In mouse plasmacytoma with the typical rcpt(12;15) chromosome translocation the c-myc oncogene is juxtaposed to one of the heavy chain genes in a head-to-head orientation. Since the relative orientations of the c-myc locus and the Ig heavy chain gene cluster on the corresponding mouse chromosomes had not been settled, it was not known whether the rearranged c-myc gene is transposed to chromosome 12 or remains on chromosome 15. To decide which of the two alternatives is correct, we separated the translocation chromosomes by fluorescence-activated chromosome sorting. The separated chromosomal fractions were hybridized with myc-specific DNA probes corresponding to the first or second/third exons in a chromosome spot assay. The results presented here indicate that the c-myc gene in mouse plasmacytoma carrying the typical translocation, as in the human Burkitt lymphoma analogous translocation, transposes to the chromosome carrying the Ig heavy chain locus. These results also establish the orientations of the Ig heavy chain locus and the c-myc locus on their normal chromosomes.
已开展实验以确定小鼠免疫球蛋白重链基因座与c-myc癌基因的相对位置。在具有典型rcpt(12;15)染色体易位的小鼠浆细胞瘤中，c-myc癌基因以头对头方向与其中一个重链基因并列。由于c-myc基因座与相应小鼠染色体上的免疫球蛋白重链基因簇的相对方向尚未确定，因此尚不清楚重排的c-myc基因是转位到12号染色体还是留在15号染色体上。为了确定这两种可能性中的哪一种是正确的，我们通过荧光激活染色体分选分离了易位染色体。在染色体斑点分析中，将分离的染色体组分与对应于第一或第二/第三外显子的myc特异性DNA探针杂交。此处呈现的结果表明，携带典型易位的小鼠浆细胞瘤中的c-myc基因，如同人类伯基特淋巴瘤类似易位一样，转位到携带免疫球蛋白重链基因座的染色体上。这些结果还确定了免疫球蛋白重链基因座和c-myc基因座在其正常染色体上的方向。
