Wood J M
J Bacteriol. 1981 Jun;146(3):895-901. doi: 10.1128/jb.146.3.895-901.1981.
L-Azetidine-2-carboxylate (AC) and 3,4-dehydro-D,L-proline (DHP) are toxic L-proline analogs that can be used to select bacterial mutants defective for L-proline transport. Mutants resistant to AC and DHP are defective for proline transport alone (putP mutants), and mutants resistant to AC but not to DHP are defective both in putP and in the closely linked proline dehydrogenase gene putA. Proline dehydrogenase oxidizes DHP but not AC, probably detoxifying the former compound. These observations were exploited in preparing an otherwise isogenic set of Escherichia coli K-12 strains with well-defined defects in the putP and putA genes. The results of this study suggest that the genetic and biochemical characteristics of proline utilization in E. coli K-12 are closely analogous to those of Salmonella typhimurium.
L-氮杂环丁烷-2-羧酸（AC）和3,4-脱氢-D,L-脯氨酸（DHP）是有毒的L-脯氨酸类似物，可用于筛选L-脯氨酸转运缺陷的细菌突变体。对AC和DHP具有抗性的突变体仅在脯氨酸转运方面存在缺陷（putP突变体），而对AC有抗性但对DHP无抗性的突变体在putP和紧密连锁的脯氨酸脱氢酶基因putA中均存在缺陷。脯氨酸脱氢酶可氧化DHP但不能氧化AC，可能是使前一种化合物解毒。利用这些观察结果制备了一组在putP和putA基因中具有明确缺陷的大肠杆菌K-12同基因菌株。这项研究的结果表明，大肠杆菌K-12中脯氨酸利用的遗传和生化特征与鼠伤寒沙门氏菌的密切相似。
