大肠杆菌K-12中的两种脯氨酸转运蛋白。
Two proline porters in Escherichia coli K-12.
作者信息
Stalmach M E, Grothe S, Wood J M
出版信息
J Bacteriol. 1983 Nov;156(2):481-6. doi: 10.1128/jb.156.2.481-486.1983.
Abstract
Escherichia coli mutants defective at putP and putA lack proline transport via proline porter I and proline dehydrogenase activity, respectively. They retain a proline uptake system (proline porter II) that is induced during tryptophan-limited growth and are sensitive to the toxic L-proline analog, 3,4-dehydroproline. 3,4-Dehydroproline-resistant mutants derived from a putP putA mutant lack proline porter II. Auxotrophic derivatives derived from putP+ or putP bacteria can grow if provided with proline at low concentration (25 microM); those derived from the 3,4-dehydroproline-resistant mutants require high proline for growth (2.5 mM). We conclude that E. coli, like Salmonella typhimurium, possesses a second proline porter that is inactivated by mutations at the proP locus.
摘要
在putP和putA基因上存在缺陷的大肠杆菌突变体，分别缺乏通过脯氨酸转运蛋白I的脯氨酸转运能力和脯氨酸脱氢酶活性。它们保留了一种脯氨酸摄取系统（脯氨酸转运蛋白II），该系统在色氨酸限制生长期间被诱导，并且对有毒的L-脯氨酸类似物3,4-脱氢脯氨酸敏感。源自putP putA突变体的3,4-脱氢脯氨酸抗性突变体缺乏脯氨酸转运蛋白II。如果在低浓度（25微摩尔）下提供脯氨酸，源自putP+或putP细菌的营养缺陷型衍生物能够生长；而源自3,4-脱氢脯氨酸抗性突变体的衍生物则需要高浓度脯氨酸（2.5毫摩尔）才能生长。我们得出结论，大肠杆菌与鼠伤寒沙门氏菌一样，拥有第二种脯氨酸转运蛋白，该转运蛋白会因proP位点的突变而失活。
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