Mizunuma T, Takahashi Y, Okuda H
J Biochem. 1981 Feb;89(2):407-10. doi: 10.1093/oxfordjournals.jbchem.a133215.
The effect of amino acids on insulin responsiveness in epididymal adipose tissue was examined. It was found that insulin-stimulated glucose oxidation in fat cells was significantly inhibited by glycine, alanine, valine, leucine, isoleucine, cysteine, methionine, lysine, phenylalanine, and proline. The effect of insulin on glucose incorporation into triglyceride is also severely diminished by these amino acids. In addition, alanine reduced the incorporation of precursors ([U-14C]glucose or [1-14C]palmitate) into triglyceride both in vitro and in vivo. The Ki values of alanine were 0.4 and 0.5 mM toward the precursors of glucose and palmitate, respectively. The mechanism of reduction of insulin responsiveness in rat adipose tissue is discussed on the basis of these results.
研究了氨基酸对附睾脂肪组织中胰岛素反应性的影响。结果发现，甘氨酸、丙氨酸、缬氨酸、亮氨酸、异亮氨酸、半胱氨酸、蛋氨酸、赖氨酸、苯丙氨酸和脯氨酸可显著抑制胰岛素刺激的脂肪细胞葡萄糖氧化。这些氨基酸还会严重削弱胰岛素对葡萄糖掺入甘油三酯的作用。此外，丙氨酸在体外和体内均降低了前体（[U-14C]葡萄糖或[1-14C]棕榈酸）掺入甘油三酯的量。丙氨酸对葡萄糖和棕榈酸前体的Ki值分别为0.4和0.5 mM。基于这些结果，讨论了大鼠脂肪组织中胰岛素反应性降低的机制。
