Inadera H, Ishikawa Y, Shirai K, Saito Y, Yoshida S
Second Department of Internal Medicine, School of Medicine, Chiba University, Japan.
Scand J Clin Lab Invest. 1993 May;53(3):225-9. doi: 10.1080/00365519309088413.
Fibroblast-like cells from subcutaneous adipose tissue and epididymal adipose tissue were prepared from rats and their proliferation and [14C]deoxy-glucose uptake and triglyceride synthesis from [14C]palmitate were investigated. The proliferation of fibroblast-like cells from subcutaneous adipose tissue was greater than that from epididymal adipose tissue. Insulin enhanced the proliferation of fibroblast-like cells derived from subcutaneous adipose tissue, but not from epididymal adipose tissue. The uptake of [14C]deoxy-glucose by fibroblast-like cells from the two sources was similar. Triglyceride synthesis from [14C]palmitate by epididymal fibroblast-like cells was higher than that by subcutaneous fibroblast-like cells. The [14C]deoxy-glucose uptake and triglyceride synthesis from [14C]palmitate by fibroblast-like cells from the two sources were not enhanced by the addition of insulin. These findings suggest the existence of different types of adipocytes in subcutaneous and epididymal adipose tissues in terms of proliferation, and show that the proliferation of fibroblast-like cells from subcutaneous adipose tissue is regulated by insulin. The triglyceride synthesis by immature adipocytes from both adipose tissues was not affected by insulin.
从大鼠制备皮下脂肪组织和附睾脂肪组织中的成纤维样细胞，并研究其增殖以及[14C]脱氧葡萄糖摄取和从[14C]棕榈酸合成甘油三酯的情况。皮下脂肪组织中成纤维样细胞的增殖大于附睾脂肪组织中的。胰岛素增强了源自皮下脂肪组织的成纤维样细胞的增殖，但未增强源自附睾脂肪组织的成纤维样细胞的增殖。来自这两个来源的成纤维样细胞对[14C]脱氧葡萄糖的摄取相似。附睾成纤维样细胞从[14C]棕榈酸合成甘油三酯的能力高于皮下成纤维样细胞。添加胰岛素并未增强来自这两个来源的成纤维样细胞对[14C]脱氧葡萄糖的摄取以及从[14C]棕榈酸合成甘油三酯的能力。这些发现表明，皮下和附睾脂肪组织中存在不同类型的脂肪细胞，在增殖方面存在差异，并表明皮下脂肪组织中成纤维样细胞的增殖受胰岛素调节。来自两种脂肪组织的未成熟脂肪细胞合成甘油三酯不受胰岛素影响。
