Jarema M A, Arndt K T, Savage M, Lu P, Miller J H
J Biol Chem. 1981 Jul 10;256(13):6544-7.
A general strategy for the insertion of nuclear spin labels throughout the sequence of a protein is illustrated with the Escherichia coli lac repressor. Examples are shown where the 19F nucleus is incorporated using 3-fluorotyrosine, as well as the selective insertion of additional protons. These selectively inserted nuclei give additional resonances in the respective NMR spectra that can be used to probe the structure and function of the proteins.
以大肠杆菌乳糖阻遏物为例，阐述了在蛋白质整个序列中插入核自旋标记的通用策略。展示了使用3-氟酪氨酸掺入19F核的示例，以及额外质子的选择性插入。这些选择性插入的核在各自的核磁共振谱中产生额外的共振峰，可用于探究蛋白质的结构和功能。
