蛋白质核磁共振谱中共振峰的基因归属：乳糖阻遏蛋白
Genetic assignment of resonances in the NMR spectrum of a protein: lac repressor.
作者信息
Jarema M A, Lu P, Miller J H
出版信息
Proc Natl Acad Sci U S A. 1981 May;78(5):2707-11. doi: 10.1073/pnas.78.5.2707.
Abstract
By using a systematic genetic approach, the resonances in the 19F NMR spectrum of 3-fluorotyrosine-substituted lac repressor protein have been assigned. The NMR data indicate that each monomer of the repressor consists of two distinct and independent domains. One domain, the NH2-terminal sixth of the primary sequence, which has been shown to be very important for DNA binding, is very mobile. The remaining COOH-terminal sequence is more rigid. Ligands of the repressor, which affect its DNA binding capability, lead to conformational changes in the COOH-terminal domain. The approach to the assignment of spectral features taken here can be extended to other systems.
摘要
通过使用系统遗传学方法，已确定了3-氟酪氨酸取代的乳糖阻遏蛋白的19F NMR谱中的共振峰。NMR数据表明，阻遏蛋白的每个单体由两个不同且独立的结构域组成。一个结构域是一级序列的NH2末端六分之一，已证明其对DNA结合非常重要，且非常灵活。其余的COOH末端序列则更为刚性。影响其DNA结合能力的阻遏蛋白配体会导致COOH末端结构域发生构象变化。此处采用的光谱特征归属方法可扩展到其他系统。
相似文献
1
Genetic assignment of resonances in the NMR spectrum of a protein: lac repressor.蛋白质核磁共振谱中共振峰的基因归属：乳糖阻遏蛋白
Proc Natl Acad Sci U S A. 1981 May;78(5):2707-11. doi: 10.1073/pnas.78.5.2707.
2
Genetic insertion of nuclear spin labels in the lac repressor.核自旋标记在乳糖阻遏物中的基因插入。
J Biol Chem. 1981 Jul 10;256(13):6544-7.
3
lac repressor-lac operator interaction: NMR observations.乳糖阻遏蛋白-乳糖操纵子相互作用：核磁共振观察结果。
Proc Natl Acad Sci U S A. 1982 Jan;79(2):218-22. doi: 10.1073/pnas.79.2.218.
4
Repressor--operator interaction in the lac operon. III. Nuclear magnetic resonance observations with altered amino-terminal DNA binding domains.乳糖操纵子中的阻遏物-操纵基因相互作用。III. 对氨基酸末端DNA结合结构域进行改变后的核磁共振观察。
J Mol Biol. 1982 Nov 5;161(3):439-57. doi: 10.1016/0022-2836(82)90248-0.
5
Sequence-specific resonance assignments in the 1H nuclear-magnetic-resonance spectrum of the lac repressor DNA-binding domain 1-51 from Escherichia coli by two-dimensional spectroscopy.通过二维光谱法对来自大肠杆菌的乳糖阻遏物DNA结合结构域1-51的1H核磁共振谱进行序列特异性共振归属。
Eur J Biochem. 1983 Dec 1;137(1-2):279-92. doi: 10.1111/j.1432-1033.1983.tb07827.x.
6
Genetic studies of the lac repressor. XII. Amino acid replacements in the DNA binding domain of the Escherichia coli lac repressor.乳糖阻遏物的遗传学研究。十二。大肠杆菌乳糖阻遏物DNA结合结构域中的氨基酸置换
J Mol Biol. 1984 Nov 25;180(1):205-12. doi: 10.1016/0022-2836(84)90438-8.
7
Origin of the asymmetrical contact between lac repressor and lac operator DNA.乳糖阻遏蛋白与乳糖操纵基因DNA之间不对称接触的起源。
J Mol Biol. 1993 Oct 5;233(3):389-99. doi: 10.1006/jmbi.1993.1519.
8
9
Secondary structure of the lac repressor DNA-binding domain by two-dimensional 1H nuclear magnetic resonance in solution.溶液中二维¹H核磁共振法测定乳糖阻遏蛋白DNA结合结构域的二级结构
Proc Natl Acad Sci U S A. 1983 Oct;80(19):5837-41. doi: 10.1073/pnas.80.19.5837.
10
Sequence of galR gene indicates a common evolutionary origin of lac and gal repressor in Escherichia coli.galR基因序列表明大肠杆菌中乳糖阻遏蛋白和半乳糖阻遏蛋白有共同的进化起源。
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2427-31. doi: 10.1073/pnas.79.8.2427.
引用本文的文献
1
A domain swapping strategy to create modular transcriptional regulators for novel topology in genetic network.一种用于创建新型遗传网络拓扑结构的模块化转录调控因子的结构域交换策略。
Biotechnol Adv. 2024 May-Jun;72:108345. doi: 10.1016/j.biotechadv.2024.108345. Epub 2024 Mar 19.
2
A systematic mutagenesis-driven strategy for site-resolved NMR studies of supramolecular assemblies.基于系统诱变的策略实现对超分子组装体的位点分辨 NMR 研究。
J Biomol NMR. 2011 Jul;50(3):229-36. doi: 10.1007/s10858-011-9513-5. Epub 2011 May 29.
3
19F NMR relaxation studies on 5-fluorotryptophan- and tetradeutero-5-fluorotryptophan-labeled E. coli glucose/galactose receptor.对5-氟色氨酸和四氘代-5-氟色氨酸标记的大肠杆菌葡萄糖/半乳糖受体的19F核磁共振弛豫研究。
J Biomol NMR. 1996 Jun;7(4):261-72. doi: 10.1007/BF00200428.
4
Informational suppression as a tool for the investigation of gene structure and function.信息抑制作为研究基因结构与功能的一种工具。
Biochem J. 1982 Apr 1;203(1):1-13. doi: 10.1042/bj2030001.
5
lac repressor-lac operator interaction: NMR observations.乳糖阻遏蛋白-乳糖操纵子相互作用：核磁共振观察结果。
Proc Natl Acad Sci U S A. 1982 Jan;79(2):218-22. doi: 10.1073/pnas.79.2.218.
6
Biosynthesis of a protein containing a nonprotein amino acid by Escherichia coli: L-2-aminohexanoic acid at position 21 in human epidermal growth factor.大肠杆菌对含非蛋白质氨基酸的蛋白质的生物合成：人表皮生长因子第21位的L-2-氨基己酸。
Proc Natl Acad Sci U S A. 1988 Sep;85(17):6237-41. doi: 10.1073/pnas.85.17.6237.
7
Isotope-detected 1H NMR studies of proteins: a general strategy for editing interproton nuclear Overhauser effects by heteronuclear decoupling, with application to phage lambda repressor.蛋白质的同位素检测1H NMR研究：通过异核去耦编辑质子间核Overhauser效应的通用策略，及其在噬菌体λ阻遏物中的应用。
Proc Natl Acad Sci U S A. 1986 Mar;83(5):1325-9. doi: 10.1073/pnas.83.5.1325.
8
lac repressor: crystallization of intact tetramer and its complexes with inducer and operator DNA.乳糖阻遏蛋白：完整四聚体及其与诱导剂和操纵基因DNA复合物的结晶
Proc Natl Acad Sci U S A. 1990 Mar;87(5):1870-3. doi: 10.1073/pnas.87.5.1870.
9
Application of site-directed mutagenesis in nuclear magnetic resonance spectroscopy.定点诱变在核磁共振光谱学中的应用。
Biophys J. 1992 Apr;62(1):116-8. doi: 10.1016/S0006-3495(92)81797-6.
本文引用的文献
1
An amino-terminal fragment of lac repressor binds specifically to lac operator.乳糖阻遏物的氨基端片段特异性结合乳糖操纵基因。
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5851-4. doi: 10.1073/pnas.75.12.5851.
2
Isolation of the lac repressor.乳糖阻遏蛋白的分离
Proc Natl Acad Sci U S A. 1966 Dec;56(6):1891-8. doi: 10.1073/pnas.56.6.1891.
3
Genetic regulatory mechanisms in the synthesis of proteins.蛋白质合成中的遗传调控机制。
J Mol Biol. 1961 Jun;3:318-56. doi: 10.1016/s0022-2836(61)80072-7.
4
A structural study of the hydrophobic box region of lysozyme in solution using nuclear Overhauser effects.利用核Overhauser效应研究溶液中溶菌酶疏水盒区域的结构
Biochemistry. 1980 Jun 10;19(12):2597-607. doi: 10.1021/bi00553a011.
5
The solution structures of tuna and horse cytochromes c.
Eur J Biochem. 1980 Feb;103(3):533-41. doi: 10.1111/j.1432-1033.1980.tb05977.x.
6
Comparison of the structures of various eukaryotic ferricytochromes c and ferrocytochromes and their antigenic differences.
Eur J Biochem. 1980 Feb;103(3):543-50. doi: 10.1111/j.1432-1033.1980.tb05978.x.
7
Inhibition of the synthesis of beta-galactosidase in Escherichia coli by 2-nitrophenyl-beta-D-fucoside.2-硝基苯基-β-D-岩藻糖苷对大肠杆菌中β-半乳糖苷酶合成的抑制作用。
J Mol Biol. 1966 Jul;18(2):339-43. doi: 10.1016/s0022-2836(66)80251-6.
8
Informational suppression.信息抑制
Annu Rev Genet. 1970;4:107-34. doi: 10.1146/annurev.ge.04.120170.000543.
9
Nuclear magnetic resonance spectroscopy of amino acids, peptides, and proteins.氨基酸、肽和蛋白质的核磁共振光谱学
Adv Protein Chem. 1970;24:447-545. doi: 10.1016/s0065-3233(08)60246-6.
10
The lac operator is DNA.乳糖操纵基因是DNA。
Proc Natl Acad Sci U S A. 1967 Dec;58(6):2415-21. doi: 10.1073/pnas.58.6.2415.
Zotero 插件