用于从人粪便中分离肉毒梭菌的选择性培养基。
Selective medium for isolation of Clostridium botulinum from human feces.
作者信息
Dezfulian M, McCroskey L M, Hatheway C L, Dowell V R
出版信息
J Clin Microbiol. 1981 Mar;13(3):526-31. doi: 10.1128/jcm.13.3.526-531.1981.
Abstract
A selective medium, Clostridium botulinum isolation (CBI) agar, was developed for the isolation of C. botulinum from human feces. This medium contains cycloserine (250 microgram/ml), sulfamethoxazole (76 microgram/ml), and trimethoprim (4 microgram/ml) as selective inhibitory agents. Qualitative tests indicated complete recovery of C. botulinum types A, B, F, and G on CBI medium. It was more difficult to recognize type G colonies on the medium because of their lack of lipase activity. Except for a few species of Clostridium, the growth of other obligate anaerobes and of the facultative anaerobes tested on CBI medium was suppressed. Quantitative studies of C. botulinum on the selective medium yielded counts comparable to those obtained on egg yolk agar control plates. Isolation of C. botulinum types A, B, and F from seeded fecal specimens was easily achieved with CBI medium. The use of CBI agar should aid the rapid isolation of C. botulinum from fecal specimens associated with foodborne and infant botulism.
摘要
一种用于从人类粪便中分离肉毒梭菌的选择性培养基——肉毒梭菌分离（CBI）琼脂被研发出来。该培养基含有环丝氨酸（250微克/毫升）、磺胺甲恶唑（76微克/毫升）和甲氧苄啶（4微克/毫升）作为选择性抑制剂。定性试验表明，A、B、F和G型肉毒梭菌在CBI培养基上能完全复苏。由于G型菌落缺乏脂肪酶活性，在该培养基上更难识别。除了少数梭菌属物种外，在CBI培养基上测试的其他专性厌氧菌和兼性厌氧菌的生长均受到抑制。在选择性培养基上对肉毒梭菌进行定量研究，得到的计数与在蛋黄琼脂对照平板上获得的计数相当。使用CBI培养基很容易从接种的粪便标本中分离出A、B和F型肉毒梭菌。CBI琼脂的使用应有助于从与食源性和婴儿肉毒中毒相关的粪便标本中快速分离肉毒梭菌。
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