Roggendorf M, Heinz F, Deinhardt F, Kunz C
J Med Virol. 1981;7(1):41-50. doi: 10.1002/jmv.1890070105.
A sensitive enzyme immunoassay is described for demonstrating specific antibodies of the IgM class to tick-borne encephalitis virus (anti-TBEV IgM). Anti-mu-coated, flat-bottomed microtiter plates are incubated with diluted patients' serum (2 hr at 37 degrees C), then with purified TBEV, and later with peroxidase-coupled anti-TBEV immunoglobulin for a further 2 hr. After washing the plates, orthophenylenediamine is added and the optical density is measured at 510 nm. At an early stage after onset of illness anti-TBEV IgM could be demonstrated up to serum dilutions of 10(-4). The specificity of the test system was shown by total inhibition of the positive reaction by preincubation of patients' sera with anti-mu. Tick-borne encephalitis (TBE) could be diagnosed serologically in 54 patients by the described method, and anti-TBEV IgM could be demonstrated in sera drawn up to 9 months after onset of illness. A peak in the number of infections occurs in the age group of 31-40 years, and most infections occur from June to October with a small peak in July.
本文描述了一种灵敏的酶免疫测定法，用于检测针对蜱传脑炎病毒的IgM类特异性抗体（抗TBEV IgM）。将包被有抗μ链的平底微量滴定板与稀释的患者血清一起孵育（37℃孵育2小时），然后与纯化的TBEV一起孵育，随后再与过氧化物酶偶联的抗TBEV免疫球蛋白一起孵育2小时。洗涤平板后，加入邻苯二胺并在510nm处测量光密度。在疾病发作后的早期，抗TBEV IgM在血清稀释至10⁻⁴时仍可检测到。通过将患者血清与抗μ链预孵育可完全抑制阳性反应，从而证明了该检测系统的特异性。通过所述方法对54例患者进行了蜱传脑炎（TBE）的血清学诊断，并且在疾病发作后长达9个月采集的血清中均可检测到抗TBEV IgM。感染人数的峰值出现在31 - 40岁年龄组，大多数感染发生在6月至10月，7月有一个小高峰。
