Chan Paul K S, Lim Pak-Leong, Liu Esther Y M, Cheung Jo L K, Leung Danny T M, Sung Joseph J Y
Centre for Emerging Infectious Diseases, Department of Microbiology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong Special Administrative Region, People's Republic of China.
J Infect Dis. 2005 Jul 1;192(1):166-9. doi: 10.1086/430615. Epub 2005 May 25.
The maturation of virus-specific immunoglobulin G avidity during severe acute respiratory syndrome-associated coronavirus infection was examined. The avidity indices were low (mean +/- SD, 30.8% +/- 11.6%) among serum samples collected < or =50 days after fever onset, intermediate (mean +/- SD, 52.1% +/- 14.1%) among samples collected between days 51 and 90, and high (mean +/- SD, 78.1% +/- 8.0%) among samples collected after day 90. Avidity indices of 40% and 55% could be considered as cutoff values for determination of recent (< or =50 days) and past (>65 days) infection, respectively. Measurement of antibody avidity can be used to differentiate primary infection from reexposure and to assess humoral responses to candidate vaccines.
研究了严重急性呼吸综合征相关冠状病毒感染期间病毒特异性免疫球蛋白G亲和力的成熟情况。在发热 onset 后≤50 天采集的血清样本中,亲和力指数较低(平均值±标准差,30.8%±11.6%);在第 51 天至 90 天之间采集的样本中,亲和力指数为中等(平均值±标准差,52.1%±14.1%);在第 90 天之后采集的样本中,亲和力指数较高(平均值±标准差,78.1%±8.0%)。40%和 55%的亲和力指数可分别作为确定近期(≤50 天)和既往(>65 天)感染的临界值。抗体亲和力的测定可用于区分初次感染和再次暴露,并评估对候选疫苗的体液反应。