鼠伤寒沙门氏菌中叠氮化物诱变代谢物的分离。

Isolation of an azide mutagenic metabolite in Salmonella typhimurium.

作者信息

Owais W M, Kleinhofs A, Ronald R C, Nilan R A

出版信息

Mutat Res. 1981 May;91(3):155-61. doi: 10.1016/0165-7992(81)90025-7.

Abstract

A scheme that employs a cation-exchange column and high-pressure liquid chromatography (HPLC) is devised to isolate and process large quantities of azide metabolite produced by S. typhimurium TA1530 strain. The mutagenic metabolite adheres strongly to the cation-exchange column, thus providing a convenient way to separate the metabolite from unreacted azide (N3-). The metabolite is very polar and only sparingly soluble in most organic solvents. Recrystallization in a methanol-carbon tetrachloride solvent system gave rise to microcrystalline material that decomposes with charring and gas evolution at 173-176 degrees C. The infrared spectrum indicates the presence of a covalently bound azide moiety.

摘要

设计了一种采用阳离子交换柱和高压液相色谱（HPLC）的方案，用于分离和处理鼠伤寒沙门氏菌TA1530菌株产生的大量叠氮化物代谢物。这种诱变代谢物强烈吸附在阳离子交换柱上，从而提供了一种将代谢物与未反应的叠氮化物（N3-）分离的便捷方法。该代谢物极性很强，在大多数有机溶剂中仅微溶。在甲醇 - 四氯化碳溶剂体系中重结晶得到微晶材料，该材料在173 - 176℃下分解并伴有炭化和气体逸出。红外光谱表明存在共价结合的叠氮基团。