Mutagenic and inhibitory properties of some new purine analogs on Salmonella typhimurium TA1530.

Mutagenic and growth-inhibitory effects of 2-amino-N6-hydroxyadenine, 2-amino-N6-methoxyadenine and 2-amino-N6-methyl-N6-hydroxyadenine were examined on S. typhimurium TA1530. All compounds showed strong mutagenic activity, and 2-amino-N6-hydroxyadenine induced mutations at a dose as low as 1 microgram/ml. 2-Amino-N6-hydroxyadenine and, to a lesser extent, 2-amino-N6-methyl-N6-hydroxyadenine (but not 2-amino-N6-methoxyadenine) exerted inhibitory effects on bacterial growth. The specificity of mutagenic action of these base analogs, as well as the target for reversion of the his- marker in TA1530, is discussed.