Pavlov Y I, Noskov V N, Lange E K, Moiseeva E V, Pshenichnov M R, Khromov-Borisov N N
Department of Genetics and Breeding, Leningrad State University, U.S.S.R.
Mutat Res. 1991 Aug;253(1):33-46. doi: 10.1016/0165-1161(91)90343-7.
The genetic activity of 2-amino-N6-hydroxyadenine or 2-amino-N-hydroxylaminopurine (AHA) and N6-hydroxyadenine or 6-N-hydroxylaminopurine (HAP) was studied in S. typhimurium, E. coli and Saccharomyces cerevisiae strains. AHA was a more potent mutagen for bacteria and a less potent mutagen for yeast than HAP. The mutagenic activity of analogs was not influenced by excision, mutagenic or double-strand DNA repair mutations. On the other hand, the uvrBdel mutation has a drastic effect on the mutagenicity and toxicity of both analogs in the Salmonella strains studied. HAP was a very potent mutagen in yeast with a low capability of inducing mitotic recombination contrary to common mutagens, possessed unique intergenic specificity and was able to induce mutations in diploids at rather high frequency.
在鼠伤寒沙门氏菌、大肠杆菌和酿酒酵母菌株中研究了2-氨基-N6-羟基腺嘌呤或2-氨基-N-羟基氨基嘌呤（AHA）以及N6-羟基腺嘌呤或6-N-羟基氨基嘌呤（HAP）的遗传活性。与HAP相比，AHA对细菌是更强效的诱变剂，而对酵母则是较弱效的诱变剂。类似物的诱变活性不受切除、诱变或双链DNA修复突变的影响。另一方面，uvrBdel突变对所研究的沙门氏菌菌株中两种类似物的诱变性和毒性有显著影响。与常见诱变剂相反，HAP在酵母中是一种非常强效的诱变剂，诱导有丝分裂重组的能力较低，具有独特的基因间特异性，并且能够以相当高的频率在二倍体中诱导突变。
