Marakusha B I, Petrovskaia V G
Zh Mikrobiol Epidemiol Immunobiol. 1981 Feb(2):37-41.
Various methods, including selection by resistance to the antibiotic mecillinam have been used to obtain Sh. flexneri mutants capable of utilizing mannitol and mannose as the sources of carbon. The mutation which determines this phenotype is localized in str-spc area and cotransduced with str A gene (28-33%). The appearance of this mutation in the initial Sh. flexneri strain 516 led to a decrease in the virulence of this strain for guinea-pigs. The restoration of its properties and the partial restoration of its virulence could be detected in Mal+ hybrids obtained by crossing with E. coli strain K12- the product of this gene may participate in the regulation of the synthesis of some pathogenicity factor.
已经使用了多种方法，包括通过对抗生素美西林的抗性进行筛选，来获得能够利用甘露醇和甘露糖作为碳源的弗氏志贺氏菌突变体。决定这种表型的突变位于str-spc区域，并与str A基因共转导（28%-33%）。这种突变在最初的弗氏志贺氏菌516菌株中的出现导致该菌株对豚鼠的毒力下降。在与大肠杆菌K12菌株杂交获得的Mal+杂种中可以检测到其特性的恢复和毒力的部分恢复——该基因的产物可能参与某些致病因子合成的调节。
