Vann W F, Schmidt M A, Jann B, Jann K
Eur J Biochem. 1981 May 15;116(2):359-64. doi: 10.1111/j.1432-1033.1981.tb05343.x.
The capsular polysaccharide was isolated from Escherichia coli 010:K5:H4; it could not be obtained from a uncapsulated (K5-) mutant. It contains N-acetylglucosamine and glucuronic acid in a molar ratio of 1:1. Acid hydrolysis of the acidic polysaccharide as well as Smith degradation and degradation by deamination of the carboxyl-reduced polysaccharide suggested that the polysaccharide is composed of a disaccharide repeating unit. The data obtained by methylation analysis and nuclear magnetic resonance spectroscopy indicated that the repeating sequence of the capsular polysaccharide is the 4-beta-glucuronyl-1,4-alpha-N-acetylglucosaminyl unit. This structure is similar to that of desulfo-heparin.
荚膜多糖是从大肠杆菌O10:K5:H4中分离得到的；无法从未包膜（K5-）突变体中获得。它含有摩尔比为1:1的N-乙酰葡糖胺和葡糖醛酸。酸性多糖的酸水解以及羧基还原多糖的史密斯降解和脱氨降解表明该多糖由二糖重复单元组成。甲基化分析和核磁共振光谱获得的数据表明，荚膜多糖的重复序列是4-β-葡糖醛酰基-1,4-α-N-乙酰葡糖胺基单元。这种结构与脱硫肝素的结构相似。
