携带大肠杆菌K-12的uvrC基因或uvrC、uvrA和ssb基因的质粒的分离。
Isolation of plasmids carrying either the uvrC or uvrC uvrA and ssb genes of Escherichia coli K-12.
作者信息
Yoakum G H, Kushner S R, Grossman L
出版信息
Gene. 1980 Dec;12(3-4):243-8. doi: 10.1016/0378-1119(80)90106-7.
A 3.4 kb PstI fragment containing the uvrC gene of Escherichia coli K-12 has been cloned into pBR322. Plasmids carrying this PstI fragment, in either orientation (pGY3233, pGY4211) relative to the cloning vehicle, complement uvrC mutants. A second plasmid (pGY3243) with a 21 kb HindIII fragment is shown to complement mutations in uvrA and ssb (single-strand binding protein). A composite plasmid (pGY4610) containing pBR322 and PstI fragments derived from pGY3233 (3.4 kb) and pGY3243 (11.05 kb) complements the uvrC, uvrA and ssb mutations.
一个含有大肠杆菌K-12 uvrC基因的3.4 kb PstI片段已被克隆到pBR322中。携带该PstI片段的质粒，相对于克隆载体以任何一种方向（pGY3233、pGY4211），都能互补uvrC突变体。另一个带有21 kb HindIII片段的质粒（pGY3243）被证明能互补uvrA和ssb（单链结合蛋白）的突变。一个包含pBR322以及源自pGY3233（3.4 kb）和pGY3243（11.05 kb）的PstI片段的复合质粒（pGY4610）能互补uvrC、uvrA和ssb突变。
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