Smith K O, Kennell W L, Lamm D L
J Immunol Methods. 1981;40(3):297-305. doi: 10.1016/0022-1759(81)90361-6.
Enzyme-linked antibody was used to treat unfixed herpesvirus-infected human fetal lung cell cultures in a mode which permitted the visualizing of local sites of infection. Foci containing as few as 20 herpesvirus-infected cells produced sufficient viral mass to be easily detectable by this method. 'Clouds' or 'plumes' of colored reaction product diffused into the substrate overlay, accumulated above and around each focus of infection and allowed quantitation of the number of foci in a culture. The number of minute centers of viral infection determined by the enzyme-linked antibody method corresponded almost exactly with values obtained by fluorescence microscopy. Quantitation of herpes simplex infectivity by focus assay was possible within only 17 h after culture inoculation, well before cytopathic effects were visible macroscopically. The technique was also applied to demonstrate measles and mumpsvirus plaques (infectious centers) in Vero cell cultures.
酶联抗体用于处理未经固定的感染疱疹病毒的人胎儿肺细胞培养物，其方式可使感染的局部部位可视化。含有少至20个感染疱疹病毒细胞的病灶产生了足够的病毒量，用这种方法很容易检测到。有色反应产物的“云”或“羽状物”扩散到覆盖的底物中，在每个感染病灶的上方和周围积聚，并可对培养物中的病灶数量进行定量。通过酶联抗体法确定的病毒感染微小中心的数量几乎与荧光显微镜获得的值完全一致。通过病灶测定法对单纯疱疹病毒感染性进行定量，在培养物接种后仅17小时内即可进行，远在肉眼可见细胞病变效应之前。该技术还用于在Vero细胞培养物中显示麻疹和腮腺炎病毒蚀斑（感染中心）。
