Whealy M E, Robbins A K, Enquist L W
Central Research and Development Department, E. I. du Pont de Nemours & Company, Wilmington, Delaware 19880-0328.
J Virol. 1989 Sep;63(9):4055-9. doi: 10.1128/JVI.63.9.4055-4059.1989.
gIII, the major envelope glycoprotein of pseudorabies virus (PRV), shares approximately 20% amino acid similarity with glycoprotein gC of herpes simplex virus type 1 (HSV-1) and HSV-2. We describe here our first experiments on the potential conservation of function between these two genes and gene products. We constructed PRV recombinants in which the gIII gene and regulatory sequences have been replaced with the entire HSV-1 gC gene and its regulatory sequences. The gC promoter functions in the PRV genome, and authentic HSV-1 gC protein is produced, albeit at a low level, in infected cells. The gC protein is present at the cell surface but cannot be detected in the PRV envelope.
gIII是伪狂犬病病毒（PRV）的主要包膜糖蛋白，与1型单纯疱疹病毒（HSV-1）和HSV-2的糖蛋白gC具有约20%的氨基酸相似性。我们在此描述了关于这两个基因及其产物之间功能潜在保守性的首次实验。我们构建了PRV重组体，其中gIII基因和调控序列已被整个HSV-1 gC基因及其调控序列所取代。gC启动子在PRV基因组中发挥作用，并且在感染细胞中能产生真正的HSV-1 gC蛋白，尽管产量较低。gC蛋白存在于细胞表面，但在PRV包膜中无法检测到。
