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本文引用的文献

1
Visualization of minute centers of viral infection in unfixed cell cultures by an enzyme-linked antibody assay.通过酶联抗体测定法对未固定细胞培养物中微小病毒感染中心进行可视化。
J Immunol Methods. 1981;40(3):297-305. doi: 10.1016/0022-1759(81)90361-6.
2
Response of weanling random-bred mice to infection with pneumonia virus of mice (PVM).断乳期随机繁殖小鼠对小鼠肺炎病毒（PVM）感染的反应。
Lab Anim Sci. 1984 Feb;34(1):35-7.
3
Reciprocal productive and restrictive virus-cell interactions of immunosuppressive and prototype strains of minute virus of mice.小鼠微小病毒免疫抑制株和原型株的相互生产性和限制性病毒-细胞相互作用
J Virol. 1983 Jun;46(3):944-55. doi: 10.1128/JVI.46.3.944-955.1983.
4
Response of weanling random-bred mice to inoculation with minute virus of mice.断奶随机繁殖小鼠对小鼠微小病毒接种的反应。
Lab Anim Sci. 1983 Feb;33(1):37-9.
5
Laboratory techniques in rabies: methods of calculation.
Monogr Ser World Health Organ. 1973(23):321-35.

一种用于鉴定和定量培养细胞中感染性小鼠细小病毒的酶免疫测定法。

An enzyme immunoassay for identification and quantification of infectious murine parvovirus in cultured cells.

作者信息

Smith A L

出版信息

J Virol Methods. 1985 Aug;11(4):321-7. doi: 10.1016/0166-0934(85)90025-4.

DOI:10.1016/0166-0934(85)90025-4
PMID:3932444
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7119543/
Abstract

An enzyme immunoassay was developed to identify and quantify infectious murine parvovirus. The assay was as sensitive as the fluorescent focus test for quantification of virus in clinical material and was less labor-intensive. Viral infectivity could be inactivated with formalin prior to performance of the enzyme immunoassay. Specificity of the reaction product was shown by neutralization of infectivity using a hyperimmune rabbit antiserum to murine parvovirus.

摘要

开发了一种酶免疫测定法来鉴定和定量感染性小鼠细小病毒。该测定法在定量临床材料中的病毒方面与荧光灶试验一样灵敏，且劳动强度较小。在进行酶免疫测定之前，病毒感染性可用福尔马林灭活。使用针对小鼠细小病毒的超免疫兔抗血清中和感染性，证明了反应产物的特异性。