Hirao Y, Yanagimachi R
J Exp Zool. 1978 Sep;205(3):433-7. doi: 10.1002/jez.1402050312.
The effects of temperature (4--37 degrees C) on sperm-egg fusion and the post-fusion events were studied. At 4--10 degrees C, acrosome-reacted spermatozoa bound to egg plasma membranes, but could not fuse with them. At 25 degrees C or above, both binding and fusion took place. The post-fusion events could occur over a broad temperature range (4--37 degrees C) but the events progressed faster with increasing temperature. An abnormal development of egg pronucleus, possibly due to an incomplete functioning of the meiotic spindle mechanism, was observed in eggs inseminated at 37 degrees C and cultured at 25 degrees C.
研究了温度（4-37摄氏度）对精卵融合及融合后事件的影响。在4-10摄氏度时，顶体反应的精子与卵质膜结合，但无法与之融合。在25摄氏度及以上时，结合和融合均会发生。融合后事件可在较宽的温度范围（4-37摄氏度）内发生，但随着温度升高，这些事件进展得更快。在37摄氏度授精并在25摄氏度培养的卵子中，观察到卵原核发育异常，这可能是由于减数分裂纺锤体机制功能不完整所致。
