Hancock R J, Cockett A T
J Immunol Methods. 1981;43(1):103-13. doi: 10.1016/0022-1759(81)90041-7.
New haemadsorption procedures for the detection of sperm bound IgG are described. Antibody-coated sperm are fixed with formaldehyde and attached by poly-L-lysine to the bases of wells on plastic plates where they can be left for several days if necessary. Sensitised indicator red cell (for direct haemadsorption) or anti-IgG sera followed by indicator red cells (for indirect haemadsorption) are then added to these wells and the presence of antibody indicated by red cell attachment to sperm. The procedure uses simple reagents and the sperm/red cell rosettes may be fixed and stained to be kept as records.
本文描述了用于检测精子结合IgG的新血细胞吸附程序。用甲醛固定抗体包被的精子，并用聚-L-赖氨酸将其附着在塑料板孔的底部，如有必要可在那里放置数天。然后将致敏指示红细胞（用于直接血细胞吸附）或抗IgG血清随后加指示红细胞（用于间接血细胞吸附）加入这些孔中，通过红细胞附着在精子上来指示抗体的存在。该程序使用简单的试剂，精子/红细胞花环可以固定和染色以保存记录。
