Hancock R J
J Immunol Methods. 1979 May 10;27(1):93-6. doi: 10.1016/0022-1759(79)90242-4.
A mixed haemadsorption procedure for the detection and localisation of spermatozoal antigens is described. Sperm are immobilised by attaching them to the bases of wells on plastic plates using poly-L-lysine and antisera and sensitised red cells are then added to these wells. The technique uses simple reagents and allows sperm/red cell rosettes to be kept as permanent records.
