Flatt P R, Bailey C J
Diabetologia. 1981 May;20(5):573-7. doi: 10.1007/BF00252768.
To investigate the effect of the ob gene in the heterozygous condition, plasma glucose and insulin responses of adult heterozygous lean (ob/+) mice were compared with mice of the homozygous lean (+/+) and homozygous obese (ob/ob) genotypes. The ob/+ mice consumed 24% more food than +/+ mice although body weights were similar. Plasma glucose and insulin concentrations were respectively 16% and 176% higher in ob/+ mice than +/+ mice in the freely fed state, and 44% and 88% higher during glucose tolerance tests. In 24 hour fasted ob/+ mice, plasma glucose concentrations were 23% higher than +/+ mice but plasma insulin concentrations were not significantly different. Arginine produced a greater insulin response (172%) and a greater fall in glycaemia (200%) in ob/+ mice. A significant difference in the hypoglycaemic effect of insulin in ob/+ and +/+ mice was not observed. These results demonstrate an effect of the ob gene on glucose homeostasis in heterozygous lean (ob/+) mice. The abnormalities were qualitatively similar but considerably less severe than those in ob/ob mice, suggesting that ob/+ mice might prove useful to study factors predisposing to inappropriate hyperglycaemia.
为了研究杂合状态下ob基因的作用，将成年杂合瘦型（ob/+）小鼠的血浆葡萄糖和胰岛素反应与纯合瘦型（+/+）和纯合肥胖型（ob/ob）基因型的小鼠进行了比较。尽管体重相似，但ob/+小鼠的食量比+/+小鼠多24%。在自由进食状态下，ob/+小鼠的血浆葡萄糖和胰岛素浓度分别比+/+小鼠高16%和176%，在葡萄糖耐量试验期间分别高44%和88%。在禁食24小时的ob/+小鼠中，血浆葡萄糖浓度比+/+小鼠高23%，但血浆胰岛素浓度无显著差异。精氨酸在ob/+小鼠中产生了更大的胰岛素反应（172%）和更大的血糖下降（200%）。未观察到ob/+和+/+小鼠胰岛素的降血糖作用有显著差异。这些结果表明ob基因对杂合瘦型（ob/+）小鼠的葡萄糖稳态有影响。这些异常在性质上相似，但比ob/ob小鼠的异常严重程度要轻得多，这表明ob/+小鼠可能有助于研究导致不适当高血糖的因素。
