Baseman J B, Hayes N S
Infect Immun. 1974 Dec;10(6):1350-5. doi: 10.1128/iai.10.6.1350-1355.1974.
Virulent Treponema pallidum organisms, extracted from infected rabbit testes, incorporated amino acids into protein. A temperature of 34 C and a pH of 7.6 were optimal for protein synthesis, which was linear during in vitro incubation for 24 h. Selective inhibition of protein synthesis by erythromycin as judged by the incorporation of radiolabeled amino acids and radioautography demonstrated that treponemes were actively synthesizing proteins. Since addition of various sera and ultrafiltrates to the basal incubation mixture did not stimulate the level of protein synthesis, it was uncertain whether treponemes synthesized protein at a maximal or endogenous rate. Based upon the size of the unlabeled amino acid pool contained in infected testicular extract, it appeared that virulent treponemes utilized the majority of amino acids for protein synthesis but at varying efficiency.
从感染兔睾丸中提取的毒力梅毒螺旋体可将氨基酸掺入蛋白质中。34℃的温度和7.6的pH值最适合蛋白质合成，在体外孵育24小时期间蛋白质合成呈线性。通过放射性标记氨基酸掺入和放射自显影判断，红霉素对蛋白质合成的选择性抑制表明螺旋体在积极合成蛋白质。由于向基础孵育混合物中添加各种血清和超滤物并未刺激蛋白质合成水平，因此不确定螺旋体是以最大速率还是内源性速率合成蛋白质。根据感染睾丸提取物中未标记氨基酸池的大小，似乎毒力螺旋体将大部分氨基酸用于蛋白质合成，但效率各不相同。
