D'Amato R F, Holmes B, Bottone E J
Crit Rev Microbiol. 1981;9(1):1-44. doi: 10.3109/10408418109104485.
"Classical" and "conventional" methods for microbial identification are still utilized in clinical microbiology laboratories; however, significant advances in methodology have taken place in the last two decades. In the transition from classical to contemporary methodologies, the reference point has changed from multistep procedures to unitary procedures with marked emphasis on standardization, speed, reproducibility, and most recently, mechanization and automation. The most evident expression of this transition is the adaptation or streamlining of classical methods in the form of "miniaturized identification systems" and their commercial availability. This review analyzes the development of the underlining principles that make the systems approach to diagnostic microbiology possible.
临床微生物学实验室仍在使用“经典”和“传统”的微生物鉴定方法；然而，在过去二十年中，方法学取得了重大进展。在从经典方法向现代方法的转变过程中，参考点已从多步骤程序转变为单一程序，显著强调标准化、速度、可重复性，以及最近的机械化和自动化。这种转变最明显的表现是以“小型化鉴定系统”的形式对经典方法进行调整或简化，并使其商业化。本综述分析了使诊断微生物学的系统方法成为可能的潜在原则的发展。
